![]() Exposed masks were processed by removal, dissolution and analysis of sampling matrix strips fixed within the mask by RT-qPCR. Demographic, clinical, and radiological data, as well as International Severe Acute Respiratory and emerging Infections Consortium (ISARIC) mortality and deterioration scores were obtained. Participants were asked to wear a modified duckbilled facemask for 30 min, followed by a nasopharyngeal swab. We have previously shown that face-mask sampling (FMS) can detect exhaled tubercle bacilli and have adapted its use to quantify exhaled SARS-CoV-2 RNA in patients admitted to hospital with Coronavirus Disease-2019 (COVID-19).Ä«etween May and December 2020, we took two concomitant FMS and nasopharyngeal samples (NPS) over two days, starting within 24 h of a routine virus positive NPS in patients hospitalised with COVID-19, at University Hospitals of Leicester NHS Trust, UK. Electronic address: to human transmission of SARS-CoV-2 is driven by the respiratory route but little is known about the pattern and quantity of virus output from exhaled breath. ![]() Electronic address: 10 Department of Respiratory Sciences, University of Leicester, United Kingdom Department of Clinical Microbiology, University Hospitals of Leicester NHS Trust, United Kingdom. 9 Department of Respiratory Sciences, University of Leicester, United Kingdom Department of Infectious Diseases and HIV Medicine, University Hospitals of Leicester NHS Trust, Leicester, United Kingdom.8 Department of Engineering, University of Leicester, United Kingdom.7 Department of Biomedical Engineering, Al-Nahrain University, Baghdad, Iraq.6 Department of Respiratory Sciences, University of Leicester, United Kingdom Department of Clinical Microbiology, University Hospitals of Leicester NHS Trust, United Kingdom.5 Department of Infectious Diseases and HIV Medicine, University Hospitals of Leicester NHS Trust, Leicester, United Kingdom.4 Department of Cardiovascular Sciences, University of Leicester, United Kingdom.3 Department of Respiratory Sciences, University of Leicester, United Kingdom.Electronic address: 2 Department of Respiratory Sciences, University of Leicester, United Kingdom Department of Infectious Diseases and HIV Medicine, University Hospitals of Leicester NHS Trust, Leicester, United Kingdom. ![]()
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